5-Amino-1MQ (Chloride) Research Standard
5-Amino-1MQ (5-Amino-1-methylquinolinium chloride) is a synthetic small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT). Unlike peptides, which are chains of amino acids, this compound is a cell-permeable small molecule that functions as an enzyme-pathway modulator. In laboratory research, it is primarily investigated for its role in regulating metabolic homeostasis, mitochondrial bioenergetics, and NAD+ salvage pathways by selectively inhibiting the methylation of nicotinamide.
Image Alt Text: Chemical structure representation of 5-Amino-1MQ (Chloride salt).
Technical Specifications
| Property | Specification |
|---|---|
| Product Name | 5-Amino-1MQ (Chloride) |
| CAS Number | Not assigned |
| Chemical Formula | C10H11ClN2 |
| Molecular Weight | 194.66 g/mol |
| Chemical Class | Small molecule (Methylquinolinium derivative) |
| Physical Appearance | Orange powder |
| Assay Purity | Refer to the batch-specific Certificate of Analysis (COA) supplied for the lot received |
| Solution Base | Sterile Water/ Benzyl Alcohol |
Mechanism of Action & Research Context
5-Amino-1MQ is studied for its ability to modulate the cellular metabolic environment through targeted enzyme inhibition.
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NNMT Inhibition: The compound acts as a substrate-targeting, selective inhibitor of NNMT. By inhibiting this enzyme, 5-Amino-1MQ prevents the methylation of nicotinamide into 1-methylnicotinamide (1-MNA).
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NAD+ Pathway Modulation: Because NNMT consumes nicotinamide and methyl groups (SAM), its inhibition is hypothesized to preserve nicotinamide for the NAD+ salvage pathway. This potentially supports intracellular NAD+ availability, which is critical for mitochondrial function and cellular energy regulation.
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Metabolic Signaling: Preclinical research utilizes this compound to investigate the suppression of adipogenesis and the improvement of insulin sensitivity in diet-induced obesity (DIO) models. It is frequently employed to map the relationship between NNMT expression and metabolic dysregulation.
Research Applications
5-Amino-1MQ is a specialized reagent essential for modern metabolic and mitochondrial research.
Primary fields of in vitro and in vivo laboratory investigation include:
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NNMT Enzyme Inhibition Studies: Quantifying the IC50 of the compound in various cell-free and cell-based enzyme assays.
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Metabolic Homeostasis Models: Evaluating the effects of NNMT inhibition on lipid metabolism, lipogenesis, and energy expenditure in adipocyte cell lines (e.g., 3T3-L1).
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NAD+ Salvage Pathway Research: Analyzing the downstream effects of nicotinamide preservation on mitochondrial efficiency and oxidative phosphorylation.
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Comparative Pharmacology: Benchmarking 5-Amino-1MQ against other metabolic modulators to delineate the specific role of NNMT in metabolic disease pathology.
Storage & Handling Guidelines
To maintain the chemical stability and experimental reliability of the compound:
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Storage Environment: Store at -20°C in a dry, cool environment for long-term stability.
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Environmental Control: Protect strictly from light and moisture, as chemical degradation can occur upon prolonged environmental exposure.
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Handling: Allow the container to equilibrate to room temperature before opening to minimize condensation. Utilize standard laboratory safety practices and appropriate PPE.
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Aseptic Technique: Maintain a sterile work environment to ensure the integrity of the compound for cell culture applications.
Related Research Compounds
Further reading: 5-Amino-1MQ research overview
Research Use Disclaimer
Research Use Only Disclaimer: This product is developed and distributed strictly as a Research Use Only (RUO) laboratory reference chemical intended exclusively for non-clinical analytical and scientific investigation. It is not an FDA-approved drug, medical treatment, dietary supplement, or food ingredient, and is strictly prohibited for human or animal consumption. Kimera Chems supplies this research material solely to qualified institutions and professional investigators for authorized laboratory research.
