5-Amino-1MQ (Chloride) Research Standard
5-Amino-1MQ (5-Amino-1-methylquinolinium chloride) is a synthetic small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT). Unlike peptides, which are chains of amino acids, this compound is a cell-permeable small molecule that functions as an enzyme-pathway modulator. In laboratory research it is used as an inhibitor of NNMT-catalysed nicotinamide methylation in enzyme and cell assays.
Physical & Handling Properties
| Property | Specification |
|---|---|
| Physical Appearance | Orange powder |
| Solution Base | Deionized Water, Benzyl Alcohol |
Mechanism of Action & Research Context
5-Amino-1MQ is studied as a selective inhibitor of a single enzyme, NNMT.
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NNMT Inhibition: The compound acts as a substrate-targeting, selective inhibitor of NNMT. By inhibiting this enzyme, 5-Amino-1MQ prevents the methylation of nicotinamide into 1-methylnicotinamide (1-MNA).
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NAD+ Pathway Modulation: Because NNMT consumes nicotinamide and methyl groups (SAM), its inhibition is hypothesized to preserve nicotinamide for the NAD+ salvage pathway.
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NNMT Expression Mapping: It is used to relate NNMT expression and activity to nicotinamide and SAM turnover in cell models.
Research Applications
5-Amino-1MQ is a reagent for NNMT enzyme research.
Primary fields of in vitro and in vivo laboratory investigation include:
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NNMT Enzyme Inhibition Studies: Quantifying the IC50 of the compound in various cell-free and cell-based enzyme assays.
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NNMT Activity in Cell Lines: Measuring 1-MNA formation and SAM consumption in cultured cell lines (e.g., 3T3-L1) when NNMT is inhibited.
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NAD+ Salvage Pathway Research: Measuring nicotinamide and NAD+ levels in cell assays when NNMT is inhibited.
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Comparative Pharmacology: Benchmarking 5-Amino-1MQ against other NNMT inhibitors by IC50 and selectivity.
Storage & Handling Guidelines
Store at controlled room temperature. Keep tightly closed. Liquid formats: do not refrigerate or freeze. Cold storage may cause the solution to cloud or precipitate. If this occurs, return to room temperature and mix until clear.
Storage guidance is a house recommendation. Analytical documentation is per-lot release testing.
Related Research Compounds
Further reading: 5-Amino-1MQ research overview
Research Use Disclaimer
Research Use Only Disclaimer: This product is labeled and sold “For Research Use Only — NOT FOR HUMAN CONSUMPTION.” It is not a drug, dietary supplement, food, cosmetic, or medical device, and it is not approved by the FDA or any other regulator for human or veterinary use. It is supplied to approved account holders as a research chemical for laboratory use and must be handled in accordance with applicable law.
