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Calculated reference model · not an experimental or batch-certified structure. All 20 atoms shown.

Model data & sources
  • The product is supplied as the chloride salt. Only the methylthioninium cation is drawn; the chloride counterion is not shown.
  • Heavy atoms only; hydrogens are omitted from the reference display.
  • Source-defined stereochemistry is checked against the calculated coordinates; no energy-minimum, crystal or solution-structure claim is made.
  • Reference molecular species only; batch identity, purity, counterions and excipients are not certified.
Formula
C16H18N3S+
Method
RDKit ETKDGv3 / MMFF94s calculated reference conformer

Methylene Blue

Nootropics

Published formats

  • Liquid Dropper 30ml/10mg per ml/300mg
  • Dry-Fill Capsule 60ct/10mg/600mg
  • Powder 5g
What is the difference between these?
Liquid Dropper
The compound already in solution, in a dropper bottle. The label gives the amount per millilitre, the bottle volume and the total.
Dry-Fill Capsule
Capsules filled with the weighed solid. The label gives the amount in each capsule and the number in the bottle.
Powder
Raw material as a solid. Nothing added and nothing portioned; the stated weight is what is in the container.
Each label gives the amount per unit and the total, so compare the totals rather than the container size.

Supplementary product details from the published catalog, checked 2026-10-07. These reference details do not establish current availability.

Pricing

Pricing and order placement are reserved for reviewed research accounts. Log in or submit an account application to view current product pricing.

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Technical Specifications

Batch COA Database ↗
CAS Registry Number61-73-4
Molecular FormulaC₁₆H₁₈ClN₃S
Molecular Weight319.85
IUPAC Designation[7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium chloride
Physical FormRefer to product documentation
Storage RequirementsRefer to product documentation
Solution VehicleDistilled Water, Potassium Sorbate, Sodium Benzoate, TEA
Other NamesMethylene Blue
Capsule Concentration10mg x 60ct
Liquid Concentration and Solution10mg/ml Distilled Water, Potassium Sorbate, Sodium Benzoate, TEA
Bulk Powder Quantity5g

Identity fields from the published product record, checked 2026-10-07. Reference information is not a batch identity certificate.

Research Overview

Methylene Blue (Redox Research Agent)

Methylene Blue (Methylthioninium chloride) is a phenothiazinium dye utilized extensively in biochemical research as a potent redox agent. It functions as an electron carrier, making it a critical tool for studying electron transport chain dynamics, mitochondrial respiration, and cellular metabolic pathways. Due to its unique ability to undergo reversible reduction, it is widely applied in in vitro models to track electron flow and examine the mechanics of oxidative stress and cellular energy metabolism.

Physical & Handling Properties

Property Specification
Appearance (Solid) Dark Brown powder
Appearance (Solution) Bright blue
Solution Base Distilled Water, Potassium Sorbate, Sodium Benzoate, TEA

Research Applications

Methylene Blue is a versatile probe used to manipulate and monitor the redox state of biological systems in controlled environments.

Primary fields of in vitro laboratory investigation include:

  • Energy Production: Studying electron transfer within the mitochondrial electron transport chain.

  • Neurobiological Investigation: Examining neuronal activity, enzymatic inhibition, and oxidative pathways in brain cell models.

  • Cell Aging: Utilizing the compound in research models to investigate cellular senescence and mitochondrial decline.

  • Cell Stress: Assessing cellular response to oxidative stress and redox-mediated signaling.

  • Oxygen Dynamics: Measuring and monitoring oxygen levels within specific cell-free and cellular models.

Storage & Handling Guidelines

Store at controlled room temperature. Keep tightly closed. Liquid formats: do not refrigerate or freeze. Cold storage may cause the solution to cloud or precipitate. If this occurs, return to room temperature and mix until clear.

Storage guidance is a house recommendation. Analytical documentation is per-lot release testing.

Related Research Compounds

Research Use Disclaimer

Research Use Only Disclaimer: This product is labeled and sold "For Research Use Only — NOT FOR HUMAN CONSUMPTION." It is not a drug, dietary supplement, food, cosmetic, or medical device, and it is not approved by the FDA or any other regulator for human or veterinary use. It is supplied to approved account holders as a research chemical for laboratory use and must be handled in accordance with applicable law.

Certificates of Analysis

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Compounds That Survive Scrutiny

Research use only. Not for human or veterinary use.