NAD⁺ (Nicotinamide Adenine Dinucleotide) Phosphate Buffered Solution
NAD⁺ (Nicotinamide Adenine Dinucleotide) Phosphate Buffered Solution is a stabilized aqueous reagent containing NAD⁺. By utilizing a phosphate-buffered system, this formulation maintains an optimal pH environment, which preserves the structural integrity and stability of the dinucleotide molecule during laboratory manipulation. As a fundamental redox coenzyme, this solution is an essential reagent for investigators exploring cellular bioenergetics, mitochondrial metabolic flux, and the activity of NAD⁺-dependent signaling enzymes in controlled in vitro environments.
Technical Specifications
| Property | Specification |
|---|---|
| Product Name | NAD⁺ Phosphate Buffered Solution |
| Concentration | 50 mg/mL |
| CAS Number | 53-84-9 |
| Chemical Formula | C21H27N7O14P2 |
| Molecular Weight | 663.43 g/mol |
| Buffer System | Phosphate-buffered aqueous solution |
| Preservative | 1% Benzyl alcohol |
| Physical Appearance | Clear, colorless to faintly yellow solution |
Mechanism of Action & Research Context
NAD⁺ serves as a central electron carrier in cellular respiration, facilitating the fundamental transfer of electrons in oxidative phosphorylation and metabolic pathways.
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Redox Cycling: It functions as a reversible coenzyme, oscillating between the oxidized state (NAD⁺) and the reduced state (NADH). This cycle is the foundation of energy metabolism in both the mitochondria and the cytosol.
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Enzymatic Cofactor Activity: Beyond respiration, NAD⁺ is a mandatory co-substrate for key regulatory enzymes, including Sirtuins (SIRTs)—which govern longevity and metabolic adaptation—and Poly(ADP-ribose) polymerases (PARPs), which are critical for DNA repair and cellular stress responses.
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Buffered Stability: The phosphate buffer system prevents pH-induced hydrolysis of the diphosphate bridge, ensuring that the concentration remains consistent throughout complex experimental protocols.
Research Applications
This solution is utilized as a specialized benchmark for high-fidelity studies in cellular biology and biochemistry.
Primary fields of in vitro laboratory investigation include:
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Mitochondrial Bioenergetics: Assessing oxidative phosphorylation rates and mitochondrial respiration flux in response to varying NAD⁺ concentrations.
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Sirtuin and PARP Signaling: Studying the activation kinetics of NAD⁺-dependent deacetylases and DNA repair enzymes.
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Metabolic Stress Models: Investigating the depletion and replenishment of NAD⁺ pools under induced oxidative or genotoxic stress conditions.
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Enzyme Kinetics: Utilizing the reagent to map the catalytic activity of NAD⁺-dependent dehydrogenases.
Storage & Handling Guidelines
Storage after receipt: refrigerate at 2-8 °C. Do not freeze. Refrigeration is a post-receipt storage condition, not a transit requirement.
Storage guidance is a house recommendation. Analytical documentation is per-lot release testing.
References
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Verdin E. (2015) NAD+ in aging, metabolism, and neurodegeneration. Science. 350(6265):1208–1213.
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Imai SI, Guarente L. (2014) NAD+ and sirtuins in aging and disease. Trends in Cell Biology. 24(8):464–471.
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Katsyuba E, Auwerx J. (2017) Modulating NAD+ metabolism as a therapeutic strategy for disease. The EMBO Journal. 36(24):3591–3605.
Analytical Documentation
Purity, identity, and composition vary by manufacturing lot. Kimera Chems does not publish a single fixed purity figure for this item; refer to the batch-specific Certificate of Analysis (COA) issued for the lot received, which reflects third-party analytical testing for that lot. Contact us if a COA for your lot is required.
Related Research Compounds
Research Use Disclaimer
Research Use Only Disclaimer: This product is developed and distributed strictly as a Research Use Only (RUO) laboratory reference chemical intended exclusively for non-clinical analytical and scientific investigation. It is not an FDA-approved drug, medical treatment, dietary supplement, or food ingredient, and is strictly prohibited for human or animal consumption. Kimera Chems supplies this research material solely to qualified institutions and professional investigators for authorized laboratory research.
