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PE-22-28 (TREK-1 Inhibitor) Research Standard
PE-22-28 is a selective peptide inhibitor of the TREK-1 (TWIK-related K+ channel 1) potassium channel. Engineered for high-specificity binding, this synthetic compound is a critical tool in neurophysiological research, allowing investigators to isolate and study the functional impact of TREK-1 channel activity. By providing a precise molecular probe to modulate potassium flux, PE-22-28 enables detailed mapping of the channel’s contribution to neuronal excitability, membrane potential, and cellular signaling in controlled in vitro environments.
Technical Specifications
| Property | Specification |
|---|---|
| Product Name | PE-22-28 |
| Chemical Formula | C₃₅H₅₅N₁₁O₉ |
| Molecular Weight | 773.90 g/mol |
| Chemical Class | Synthetic Peptide / TREK-1 Inhibitor |
| Physical Appearance | White to off-white lyophilized solid |
| Assay Purity | Refer to the batch-specific Certificate of Analysis (COA) supplied for the lot received |
Mechanism of Action & Research Context
PE-22-28 functions as a site-specific ligand that modulates the conductance of the TREK-1 potassium channel, which is a member of the two-pore domain potassium (K2P) channel family.
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Channel Binding: The peptide is designed to interact specifically with the TREK-1 protein complex. By occupying or modulating the binding site of this channel, it alters potassium permeability across the cell membrane, which is essential for studying resting membrane potential and cellular excitability.
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Structural Specificity: Research centers on the molecular “fit” of the peptide within the channel pocket. Investigators utilize this interaction to study the structure-activity relationship (SAR) of peptide-channel inhibitors and to delineate the specific domains of TREK-1 that regulate ion flux.
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Modulation of Neuronal Signaling: As potassium flux is a major determinant of neuronal firing thresholds, PE-22-28 acts as a pharmacological probe to investigate how TREK-1 inhibition affects signal propagation and synaptic integration in neuronal tissue models.
Research Applications
PE-22-28 is a specialized reagent essential for electrophysiological and molecular neurobiology research.
Primary fields of in vitro laboratory investigation include:
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Channel Binding Assays: Measuring the affinity and kinetics of peptide-channel attachment, often using specialized binding site mapping techniques.
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Structure-Function Studies: Examining how modifications to the peptide sequence influence its inhibitory potency against TREK-1.
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Specificity Testing: Evaluating the selectivity of PE-22-28 by testing its binding activity against other similar potassium channels (e.g., TREK-2, TRAAK) to ensure target fidelity.
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Neuronal Excitability Modeling: Observing the downstream effects of TREK-1 inhibition on the membrane potential and firing patterns of cultured neurons.
Storage & Handling Guidelines
To maintain the chemical stability and experimental reliability of the peptide:
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Storage Environment: Keep lyophilized powder in a cool, dry place. For long-term stability, store in a freezer (typically -20 °C).
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Reconstitution: If reconstituted into a solution, keep the sample refrigerated (2–8 °C) and minimize freeze-thaw cycles.
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Light Protection: Store away from direct light to prevent degradation of the peptide sequence.
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Container Integrity: Ensure the cap is closed tightly after every use to prevent atmospheric exposure. Use only sterile laboratory tools when handling to avoid cross-contamination.
Related Research Compounds
Research Use Disclaimer
Research Use Only Disclaimer: This product is developed and distributed strictly as a Research Use Only (RUO) laboratory reference chemical intended exclusively for non-clinical analytical and scientific investigation. It is not an FDA-approved drug, medical treatment, dietary supplement, or food ingredient, and is strictly prohibited for human or animal consumption. Kimera Chems supplies this research material solely to qualified institutions and professional investigators for authorized laboratory research.
